Chemical ligation of epoxide-containing fusicoccins and peptide fragments guided by 14-3-3 protein.
نویسندگان
چکیده
The template effect of 14-3-3 protein on the chemical ligation of fusicoccin derivatives containing an epoxide group and the pentapeptide QSYDC was investigated. HPLC analysis of the epoxide-opening reaction demonstrated that 14-3-3ζ protein improves the yield of the conjugate product compared to the protein-free control.
منابع مشابه
Optimisation of chemical protein cleavage for erythropoietin semi-synthesis using native chemical ligation† †Electronic supplementary information (ESI) available: MS and LC-MS characterisation for thioester 2 and the M54L EPO mutant. See DOI: 10.1039/b811501j Click here for additional data file.
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ورودعنوان ژورنال:
- Molecular bioSystems
دوره 9 5 شماره
صفحات -
تاریخ انتشار 2013